Description
The EL4.IL-2 cells, a distinct subline of EL4, which has been derived from the thymus of a mouse that was affected by lymphoma, expresses the unique characteristics of T lymphoblasts. This makes them an exceptional model for exploring the intricacies of T-cell lymphomas and various aspects of the immune system. A key attribute of the EL4.IL-2 cell line is its exceptional capability to produce interleukin-2 (IL-2), an essential cytokine that plays a pivotal role in immune responses. When these cells are exposed to phorbol-12-myristate-13-acetate (PMA), they exhibit a remarkable proficiency in IL-2 production. The levels can reach up to 2500 units/ml after a 24-hour cultivation period. This potent ability to generate high amounts of IL-2 makes EL4.IL-2 cells an ideal candidate for investigating the various roles and functionalities of IL-2 in different biological contexts. The origin of EL4.IL-2 cells from the thymus, a crucial organ for T-cell maturation, confers upon them properties that closely resemble those of lymphoblasts. This aspect, combined with their lymphoma background, provides a unique opportunity to examine the behaviors and characteristics of lymphoblast-like cells in a controlled experimental environment. Moreover, EL4.IL-2 cells are characterized by a moderate doubling time, ranging approximately between 15 to 20 hours. The predictable and consistent growth pattern of these cells is an essential factor that enhances the efficiency and reliability of various research studies